Article
Unusual ring D fixation by three crucial residues promotes phycoviolobilin formation in the DXCF-type cyanobacteriochrome without the second Cys.
The Biochemical journal - 12 Mar 2021
Fushimi Keiji, Narikawa Rei
Abstract excerpt
Cyanobacteriochromes are linear tetrapyrrole-binding photoreceptors produced by cyanobacteria. Their chromophore-binding GAF domains are categorized into many lineages. Among them, dual Cys-type cyanobacteriochrome GAF domains possessing not only a highly conserved 'first Cys' but also a 'second Cys' are found from multiple lineages. The first Cys stably attaches to C31 of the A-ring, while the second Cys mostly...
Topics
- Cyanobacteria
- Cysteine
- Mutagenesis, Site-Directed
- Mutation
- Photoreceptors, Microbial
- Phycobilins
- Phytochrome
- Protein Conformation
- Protein Domains