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Article

Toward the Establishment of a Single Standard Curve for Quantification of Trypanosoma cruzi Natural Populations Using a Synthetic Satellite Unit DNA Sequence.

The Journal of molecular diagnostics : JMD - 1 May 2021

Muñoz-Calderón Arturo, Silva-Gomes Natalia Lins, Apodaca Sofia, Alarcón de Noya Belkisyolé, Díaz-Bello Zoraida, Souza Leticia Rocha Quintino, Costa Alexandre Dias Tavares, Britto Constança, Moreira Otacilio Cruz, Schijman Alejandro G

Abstract excerpt

Accurate diagnostic tools and surrogate markers of parasitologic response to treatment are needed for managing Chagas disease. Quantitative real-time PCR (qPCR) is used for treatment monitoring, but variability in copy dosage and sequences of molecular target genes among different Trypanosoma cruzi strains limit the precision of quantitative measures. To improve qPCR quantification accuracy, we designed and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 33549859
DOI: 10.1016/j.jmoldx.2021.01.007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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