Article
[A method of determining the level of provitamin D in Saccharomyces cerevisiae with altered sterol metabolism].
Prikladnaia biokhimiia i mikrobiologiia - 1 Jan 2000
Chichikalova M M, Zhakovskaia Z A, Moseĭchuk A V, Mikhalova N P, V'iunov K A
Abstract excerpt
A method is proposed that allows to choose among the polyene-resistant mutants of the yeasts Saccharomyces cerevisiae with changed biosynthesis of ergosterol promising producers of provitamin D4 and structural analogs of provitamin D3. The method involves both UV-spectrophotometry and thin-layer chromatography. The results obtained are supported by the data of mass-spectrometry of sterols.
Topics
- Chromatography, Thin Layer
- Drug Resistance, Microbial
- Mutation
- Prodrugs
- Saccharomyces cerevisiae
- Spectrophotometry, Ultraviolet
- Sterols
- Vitamin D