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Article

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping.

Journal of visualized experiments : JoVE - 27 Oct 2020

Estrada-Gutierrez Guadalupe, Bravo-Flores Eyerahi, Ortega-Castillo Veronica, Flores-Rueda Veronica, Mancilla-Herrera Ismael, Espino Y Sosa Salvador, Sánchez-Martínez Maribel, Perichart-Perera Otilia, Solis-Paredes Mario

Abstract excerpt

Visceral adipose tissue (VAT) is an active metabolic organ composed mainly of mature adipocytes and stromal vascular fraction (SVF) cells, which release different bioactive molecules that control metabolic, hormonal, and immune processes; currently, it is unclear how these processes are regulated within the adipose tissue. Therefore, the development of methods evaluating the contribution of each cell population...

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Identifiers

PMID: 33191941
DOI: 10.3791/61884

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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