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Assessment of the direct quantitation of SARS-CoV-2 by droplet digital PCR

30 Oct 2020

Abstract excerpt

Droplet digital PCR (ddPCR) is a sensitive and reproducible technology widely used for quantitation of several viruses. The aim of this study was to evaluate the 2019-nCoV CDC ddPCR Triplex Probe Assay (BioRad) performance, comparing the direct quantitation of SARS-CoV-2 on nasopharyngeal swab with the procedure applied to the extracted RNA. Moreover, two widely used swab types were compared (UTM 3 mL and ESwab 1...

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PMID: 33127953
DOI: 10.1038/s41598-020-75958-x

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