Generating and characterizing single- and multigene mutants of the Rubisco small subunit family in Arabidopsis.

doi:10.1093/jxb/eraa316

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Article

Generating and characterizing single- and multigene mutants of the Rubisco small subunit family in Arabidopsis.

Journal of experimental botany - 7 Oct 2020

Khumsupan Panupon, Kozlowska Marta A, Orr Douglas J, Andreou Andreas I, Nakayama Naomi, Patron Nicola, Carmo-Silva Elizabete, McCormick Alistair J

Abstract excerpt

The primary CO2-fixing enzyme Rubisco limits the productivity of plants. The small subunit of Rubisco (SSU) can influence overall Rubisco levels and catalytic efficiency, and is now receiving increasing attention as a potential engineering target to improve the performance of Rubisco. However, SSUs are encoded by a family of nuclear rbcS genes in plants, which makes them challenging to engineer and study. Here we...

Topics

Arabidopsis
CRISPR-Cas Systems
Gene Knockout Techniques
Mutation
Phenotype
Ribulose-Bisphosphate Carboxylase

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PMID: 32734287
DOI: 10.1093/jxb/eraa316

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