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Article

Validation of a quantitative PCR assay for the detection of 2 Flavobacterium columnare genomovars.

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc - 1 May 2020

Gibbs Gordon D, Griffin Matthew J, Mauel Michael J, Lawrence Mark L

Abstract excerpt

Flavobacterium columnare is the causative agent of columnaris disease in a variety of fish hosts. Using modifications to previously established protocols, a quantitative PCR (qPCR) assay was validated for the detection of 2 predominant F. columnare genomovars. The oligonucleotide primer and probe combination was designed to amplify a 203-bp region of the chondroitin AC lyase gene (GenBank AY912281) of F....

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Identifiers

PMID: 32310022
DOI: 10.1177/1040638720915760
PMCID: PMC7377616

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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