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Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

7 Apr 2020

Abstract excerpt

Abstract Background Crocus sativusis a recalcitrant plant for genetic transformation and genetic improvement, largely due to difficulties inAgrobacteriummediated transformation and vegetative reproduction. Effective genome editing requires proficient callus production and an efficient method to deliver Cas9 and sgRNAs into the plant. Here, we demonstrateAgrobacterium-mediated transformation of saffron. Further,...

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PMID: 32280363
DOI: 10.1186/s13007-020-00589-2

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