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Establishment of human iPSC line from patient of Indian ethnicity carrying homozygous CD8/9 (+G) beta thalassemia mutation.

Stem cell research - 1 Apr 2020

Tembe Shruti, Fernandes Sophia, Khan Nikhat, Melinkeri Sameer, Kale Vaijayanti, Limaye Lalita

Abstract excerpt

This study shows generation of iPSCs from peripheral blood mononuclear cells (PBMNCs) of a male patient having homozygous CD 8/9 (+G) beta thalassemia (major) mutation. Cells were nucleofected with episomal vectors containing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (dominant negative p53 mutation). Cell line exhibited presence of pluripotency markers by immunofluorescence, flow-cytometry and PCR. The plasmids...

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PMID: 32278313
DOI: 10.1016/j.scr.2020.101772

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