Article
A two-step method for identifying photopigment opsin and rhodopsin gene sequences underlying human color vision phenotypes.
Molecular vision - 1 Jan 2020
Atilano Shari R, Kenney M Cristina, Briscoe Adriana D, Jameson Kimberly A
Abstract excerpt
Purpose: To present a detailed, reliable long range-PCR and sequencing (LR-PCR-Seq) procedure to identify human opsin gene sequences for variations in the long wavelength-sensitive (OPN1LW), medium wavelength-sensitive (OPN1MW), short wavelength-sensitive (OPN1SW), and rhodopsin (RHO) genes. Methods: Color vision was assessed for nine subjects using the Farnsworth-Munsell 100 hue test, Ishihara pseudoisochromatic...
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