Article
A 124-plex Microhaplotype Panel Based on Next-generation Sequencing Developed for Forensic Applications.
Scientific reports - 6 Feb 2020
Pang Jing-Bo, Rao Min, Chen Qing-Feng, Ji An-Quan, Zhang Chi, Kang Ke-Lai, Wu Hao, Ye Jian, Nie Sheng-Jie, Wang Le
Abstract excerpt
Microhaplotypes are an emerging type of forensic genetic marker that are expected to support multiple forensic applications. Here, we developed a 124-plex panel for microhaplotype genotyping based on next-generation sequencing (NGS). The panel yielded intralocus and interlocus balanced sequencing data with a high percentage of effective reads. A full genotype was determined with as little as 0.1 ng of input DNA....
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