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Optimization of 16S rRNA gene analysis for use in the diagnostic clinical microbiology service

23 Jan 2020

Abstract excerpt

Broad-range amplification and sequencing of the 16S rRNA gene, directly from clinical samples, is a method that potentially allows detection of any cultivable or non-cultivable bacteria. However, the method is prone to false positive results due to PCR contamination. Another concern is the human DNA abundance compared to bacterial DNA in samples from sterile sites. Those factors may decrease the sensitivity and...

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PMID: 31982454
DOI: 10.1016/j.mimet.2020.105854

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