Oropouche virus cases identified in Ecuador using an optimised qRT-PCR informed by metagenomic sequencing

Abstract excerpt

Oropouche virus (OROV) is responsible for outbreaks of Oropouche fever in parts of South America. We recently identified and isolated OROV from a febrile Ecuadorian patient, however, a previously published qRT-PCR assay did not detect OROV in the patient sample. A primer mismatch to the Ecuadorian OROV lineage was identified from metagenomic sequencing data. We report the optimisation of an qRT-PCR assay for the...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Vector-Borne Animal Diseases
• Share to Vector-borne infectious diseases
• Share to Viral Infections and Vectors