Generation of targeted homozygosity in the genome of human induced pluripotent stem cells.

doi:10.1371/journal.pone.0225740

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Article

Generation of targeted homozygosity in the genome of human induced pluripotent stem cells.

PloS one - 1 Jan 2019

Yoshimura Yasuhide, Yamanishi Ayako, Kamitani Tomo, Kim Jin-Soo, Takeda Junji

Abstract excerpt

When loss of heterozygosity (LOH) is correlated with loss or gain of a disease phenotype, it is often necessary to identify which gene or genes are involved. Here, we developed a region-specific LOH-inducing system based on mitotic crossover in human induced pluripotent stem cells (hiPSCs). We first tested our system on chromosome 19. To detect homozygous clones generated by LOH, a positive selection cassette was...

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Identifiers

PMID: 31805151
DOI: 10.1371/journal.pone.0225740
PMCID: PMC6894808

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.