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Article

Unexpected mitochondrial genome diversity revealed by targeted single-cell genomics of heterotrophic flagellated protists.

Nature microbiology - 1 Jan 2020

Wideman Jeremy G, Monier Adam, Rodríguez-Martínez Raquel, Leonard Guy, Cook Emily, Poirier Camille, Maguire Finlay, Milner David S, Irwin Nicholas A T, Moore Karen, Santoro Alyson E, Keeling Patrick J, Worden Alexandra Z, Richards Thomas A

Abstract excerpt

Most eukaryotic microbial diversity is uncultivated, under-studied and lacks nuclear genome data. Mitochondrial genome sampling is more comprehensive, but many phylogenetically important groups remain unsampled. Here, using a single-cell sorting approach combining tubulin-specific labelling with photopigment exclusion, we sorted flagellated heterotrophic unicellular eukaryotes from Pacific Ocean samples. We...

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PMID: 31768028
DOI: 10.1038/s41564-019-0605-4

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