Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis

Abstract excerpt

Diagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing is an emerging approach that allows pathogen identification in challenging situations, as evidenced by a clinical case. We extracted DNA from the surgically-removed frozen valve tissue from a patient with suspected...

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