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From Culture-Negative to DNA-Positive: The Molecular Revolution in Infective Endocarditis Diagnosis

23 May 2025

Abstract excerpt

Infective endocarditis (IE) remains a diagnostic challenge, particularly in cases where microbiological diagnosis is not established. Advances in molecular diagnostics have expanded the ability to identify causative pathogens beyond traditional culture-based methods. This review explores the role of molecular assays, including pathogen-specific PCR, multiplex PCR, broad-range PCR, and shotgun metagenomic...

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PMID: 40559526
DOI: 10.3390/pathogens14060518

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