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Development of a qPCR platform for quantification of the five bacteriophages within bacteriophage cocktail 2 (BFC2)

25 Sept 2019

Abstract excerpt

To determine phage titers accurately, reproducibly and in a non-laborious and cost-effective manner, we describe the development of a qPCR platform for molecular quantification of five phages present in bacteriophage cocktail 2 (BFC2). We compared the performance of this molecular approach, with regard to quantification and reproducibility, with the standard culture-based double agar overlay method (DAO). We...

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PMID: 31554892
DOI: 10.1038/s41598-019-50461-0

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