Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Detection of viable but non-culturable Pseudomonas aeruginosa in cystic fibrosis by qPCR: a validation study

1 Dec 2018

Abstract excerpt

BACKGROUND: Routine culture-based diagnosis of Pseudomonas aeruginosa lung infection in Cystic Fibrosis (CF) patients can be hampered by the phenotypic variability of the microorganism, including its transition to a Viable But Non-Culturable (VBNC) state. The aim of this study was to validate an ecfX-targeting qPCR protocol developed to detect all viable P. aeruginosa bacteria and to identify VBNC forms in CF...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacterial biofilms and quorum sensing
Share to Cystic Fibrosis Research Advances
Share to Vibrio bacteria research studies

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 30587160
DOI: 10.1186/s12879-018-3612-9

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Detection of viable but non-culturable Pseudomonas aeruginosa in cystic fibrosis by qPCR: a validation studyPMID 30587160Read ↗

Proposal of a quantitative PCR-based protocol for an optimal Pseudomonas aeruginosa detection in patients with cystic fibrosisSemantic proximity Read ↗

Comparison of the sensitivity of culture, PCR and quantitative real-time PCR for the detection of Pseudomonas aeruginosain sputum of cystic fibrosis patientsSemantic proximity Read ↗

The Microbiology of Non-aeruginosa Pseudomonas Isolated From Adults With Cystic Fibrosis: Criteria to Help Determine the Clinical Significance of Non-aeruginosa Pseudomonas in CF Lung PathologySemantic proximity Read ↗

Reliability of Quantitative Real-Time PCR for Bacterial Detection in Cystic Fibrosis Airway SpecimensSemantic proximity Read ↗

RNA-based qPCR as a tool to quantify and to characterize dual-species biofilmsSemantic proximity Read ↗

Analysis of Culture-Dependent versus Culture-Independent Techniques for Identification of Bacteria in Clinically Obtained Bronchoalveolar Lavage FluidSemantic proximity Read ↗

Culture-Independent Identification of Nontuberculous Mycobacteria in Cystic Fibrosis Respiratory SamplesSemantic proximity Read ↗

The microorganisms in chronically infected end-stage and non-end-stage cystic fibrosis patientsSemantic proximity Read ↗

Molecular identification and genotyping of Pseudomonas aeruginosa isolated from cystic fibrosis and non-cystic fibrosis patients with bronchiectasis.Semantic proximity Read ↗

EnhancedIn VitroFormation and Antibiotic Resistance of Nonattached Pseudomonas aeruginosa Aggregates through Incorporation of Neutrophil ProductsSemantic proximity Read ↗

Methods for Extraction and Detection of Pf Bacteriophage DNA from the Sputum of Patients with Cystic FibrosisSemantic proximity Read ↗

Use of Real-Time PCR with Multiple Targets To Identify <i>Pseudomonas aeruginosa</i> and Other Nonfermenting Gram-Negative Bacilli from Patients with Cystic FibrosisSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.