Article
Chloroplast sequence variation and the efficacy of peptide nucleic acids for blocking host amplification in plant microbiome studies.
Microbiome - 18 Aug 2018
Fitzpatrick Connor R, Lu-Irving Patricia, Copeland Julia, Guttman David S, Wang Pauline W, Baltrus David A, Dlugosch Katrina M, Johnson Marc T J
Abstract excerpt
BACKGROUND: The ability to efficiently characterize microbial communities from host individuals can be limited by co-amplification of host organellar sequences (mitochondrial and/or plastid), which share a common ancestor and thus sequence similarity with extant bacterial lineages. One promising approach is the use of sequence-specific peptide nucleic acid (PNA) clamps, which bind to, and block amplification of,...
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