Article
Multistep mutational transformation of a protein fold through structural intermediates.
Protein science : a publication of the Protein Society - 1 Oct 2018
Kumirov Vlad K, Dykstra Emily M, Hall Branwen M, Anderson William J, Szyszka Taylor N, Cordes Matthew H J
Abstract excerpt
New protein folds may evolve from existing folds through metamorphic evolution involving a dramatic switch in structure. To mimic pathways by which amino acid sequence changes could induce a change in fold, we designed two folded hybrids of Xfaso 1 and Pfl 6, a pair of homologous Cro protein sequences with ~40% identity but different folds (all-α vs. α + β, respectively). Each hybrid, XPH1 or XPH2, is 85%...
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