Failure of a multiplex polymerase chain reaction assay to detect IMP-27 in a clinical isolate of Morganella morganii.

doi:10.1016/j.diagmicrobio.2018.06.011

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Article

Failure of a multiplex polymerase chain reaction assay to detect IMP-27 in a clinical isolate of Morganella morganii.

Diagnostic microbiology and infectious disease - 1 Nov 2018

Walkty Andrew, Adam Heather, Tran Vanessa, Alexander David C, Boyd David, Bharat Amrita, Karlowsky James

Abstract excerpt

A case of an IMP-27-positive Morganella morganii isolate is reported, where the carbapenemase enzyme was demonstrated by whole genome sequencing. Carbapenemase detection using a multiplex PCR assay was negative due to mutations in the primer binding site. This case serves to illustrate the limitations of multiplex PCR for carbapenemase detection.

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PMID: 30025967
DOI: 10.1016/j.diagmicrobio.2018.06.011

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.