Article
A new method for detection of single nucleotide polymorphism in a female reproduction-associated gene, tmigd1, of Anas platyrhynchos using a strip biosensor with gold nanoparticles.
Poultry science - 1 Oct 2018
Lin Chih-Wei, Kuo Jui-Chao, Liu Hsiao-Lung, Cheng Yu-Shin, Huang Hsiu-Lin
Abstract excerpt
In this study, we first reported a lateral flow assay combined with primer extension (PEXT) and gold nanoparticles for single-nucleotide polymorphism (SNP) genotyping of the tmigd1 gene of the Tsaiya ducks (Anas platyrhynchos), which has the advantages of simplicity of operation, cost-effectiveness, and time-saving. Gold nanoparticles were tailed with thiol-thymine oligodeoxyribonucleotides (thiol-(dT)30) using...
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