Article
Characteristic and influencing factors of Taqman genotyping calling error.
Journal of clinical laboratory analysis - 1 Nov 2018
Yu Songcheng, Li Xing, Liu Xinxin, Wang Yan, Yu Fei, Xue Yuan, Mao Zhenxing, Wang Chongjian, Li Wenjie
Abstract excerpt
BACKGROUND: Taqman fluorescent probe was frequently applied in single nucleotide polymorphism (SNP) genotyping. However, the characteristic of calling error and the influencing factors remain unclear. METHOD: Calling errors of Taqman genotyping was evaluated systematically based on Mendelian inheritance. Twenty-two SNPs were genotyped by Taqman probe for 419 pedigrees. Mendelian genetic errors were counted for...
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