Article
Detection and quantification of hepatitis E virus in the absence of IgG and IgM anti-HEV in HIV-positive patients.
Journal of applied microbiology - 1 Oct 2018
Salvio A L, Lopes A O, Almeida A J, Gardinali N R, Lima L R P, de Oliveira J M, Sion F S, Ribeiro L C P, Pinto M A, de Paula V S
Abstract excerpt
AIMS: To improve RT-qPCR with an internal control and a synthetic standard curve to detect HEV in HIV co-infected patients. METHODS AND RESULTS: A single-stranded RNA (ssRNA) and a double-stranded DNA (dsDNA) synthetic curve were designed, compared to the international reference panel for HEV genotypes, and tested to quantify and detect a reference panel for HEV genotypes. The detection limit of the RNA synthetic...
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