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Article

Optimization of subculture and DNA extraction steps within the whole genome sequencing workflow for source tracking of Salmonella enterica and Listeria monocytogenes.

Journal of microbiological methods - 1 Aug 2018

Gimonet Johan, Portmann Anne-Catherine, Fournier Coralie, Baert Leen

Abstract excerpt

This work shows that an incubation time reduced to 4-5 h to prepare a culture for DNA extraction followed by an automated DNA extraction can shorten the hands-on time, the turnaround time by 30% and increase the throughput while maintaining the WGS quality assessed by high quality Single Nucleotide Polymorphism analysis.

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Identifiers

PMID: 29920304
DOI: 10.1016/j.mimet.2018.06.005

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