Article
Tm-Shift Detection of Dog-Derived Ancylostoma ceylanicum and A. caninum.
BioMed research international - 1 Jan 2018
Fu Yeqi, Wang Mingwei, Yan Xinxin, Abdullahi Auwalu Yusuf, Hang Jianxiong, Zhang Pan, Huang Yue, Liu Yunqiu, Sun Yongxiang, Ran Rongkun, Li Guoqing
Abstract excerpt
To develop a Tm-shift method for detection of dog-derived Ancylostoma ceylanicum and A. caninum, three sets of primers were designed based on three SNPs (ITS71, ITS197, and ITS296) of their internal transcribed spacer 1 (ITS1) sequences. The detection effect of the Tm-shift was assessed through the stability, sensitivity, accuracy test, and clinical detection. The results showed that these three sets of primers...
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