Article
Development of T m -shift genotyping method for detection of cat-derived Giardia lamblia.
Parasitology research - 1 Apr 2017
Pan Weida, Fu Yeqi, Abdullahi Auwalu Yusuf, Wang Mingwei, Shi Xianli, Yang Fang, Yu Xingang, Yan Xinxin, Zhang Pan, Hang Jianxiong, Li Guoqing
Abstract excerpt
To develop T m -shift genotyping method for detection of cat-derived Giardia lamblia, two sets of primers with two GC-rich tails of unequal length attached to their 5'-end were designed according to two SNPs (BG434 and BG170) of β-giardin (bg) gene, and specific PCR products were identified by inspection of a melting curve on real-time PCR thermocycler. A series of experiments on the stability, sensitivity, and...
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