Article
Serine 105 and 120 are important phosphorylation sites for porcine reproductive and respiratory syndrome virus N protein function.
Veterinary microbiology - 1 Jun 2018
Chen Yao, Xing Xiulin, Li Qi, Feng Songlin, Han Xiaoliang, He Shuyi, Zhang Guihong
Abstract excerpt
The nucleocapsid (N) protein is the most abundant protein of porcine reproductive and respiratory syndrome virus (PRRSV). It has been shown to be multiphosphorylated. However, the phosphorylation sites are still unknown. In this study, we used liquid chromatography tandem mass spectrometry (LC-MS/MS) to analyze the phosphorylation sites of N protein expressed in Sf9 cells. The results showed that N protein...
Topics
- Animals
- Chromatography, Liquid
- Down-Regulation
- Interleukin-10
- Kinetics
- Macrophages, Alveolar
- Mutation
- Nucleocapsid Proteins
- Phosphorylation
- Porcine Reproductive and Respiratory Syndrome
- Porcine respiratory and reproductive syndrome virus
- Serine
- Sf9 Cells