Refined protocols of tamoxifen injection for inducible DNA recombination in mouse astroglia.

doi:10.1038/s41598-018-24085-9

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Community

Back to search

Article

Refined protocols of tamoxifen injection for inducible DNA recombination in mouse astroglia.

Scientific reports - 12 Apr 2018

Jahn Hannah M, Kasakow Carmen V, Helfer Andreas, Michely Julian, Verkhratsky Alexei, Maurer Hans H, Scheller Anja, Kirchhoff Frank

Abstract excerpt

Inducible DNA recombination of floxed alleles in vivo by liver metabolites of tamoxifen (TAM) is an important tool to study gene functions. Here, we describe protocols for optimal DNA recombination in astrocytes, based on the GLAST-CreERT2/loxP system. In addition, we demonstrate that quantification of genomic recombination allows to determine the proportion of cell types in various brain regions. We analyzed the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Astrocytes
Share to Cerebellum
Share to Chromatography, Liquid
Share to Receptors, AMPA

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Receptors, GABA-B

Share to RNA, Untranslated

Share to Tamoxifen

Share to Tandem Mass Spectrometry

Source

Open in PubMed Open full text

Identifiers

PMID: 29651133
DOI: 10.1038/s41598-018-24085-9
PMCID: PMC5897555

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.