Article
Monitoring Shedding of Five Genotypes of RotaTeq Vaccine Viruses by Genotype-Specific Real-Time Reverse Transcription-PCR Assays.
Journal of clinical microbiology - 1 Jun 2018
Higashimoto Yuki, Ihira Masaru, Miyazaki Yu, Kuboshiki Ayumi, Yoshinaga Sayaka, Hiramatsu Hiroyuki, Suzuki Ryota, Miyata Masafumi, Miura Hiroki, Komoto Satoshi, Yukitake Jun, Taniguchi Koki, Kawamura Yoshiki, Yoshikawa Tetsushi
Abstract excerpt
RotaTeq (RV5) is a widely used live attenuated pentavalent rotavirus (RV) vaccine. Although fecal shedding of RV vaccine strains persists for long time periods, it is unclear how each vaccine strain replicates in intestinal tissue and is excreted in stool. To examine this issue, we established RV5 genotype-specific real-time reverse transcription-PCR (RT-PCR) assays. Five real-time RT-PCR assays were designed for...
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