Article
Precise Cas9 targeting enables genomic mutation prevention.
Proceedings of the National Academy of Sciences of the United States of America - 3 Apr 2018
Chavez Alejandro, Pruitt Benjamin W, Tuttle Marcelle, Shapiro Rebecca S, Cecchi Ryan J, Winston Jordan, Turczyk Brian M, Tung Michael, Collins James J, Church George M
Abstract excerpt
Here, we present a generalized method of guide RNA "tuning" that enables Cas9 to discriminate between two target sites that differ by a single-nucleotide polymorphism. We employ our methodology to generate an in vivo mutation prevention system in which Cas9 actively restricts the occurrence of undesired gain-of-function mutations within a population of engineered organisms. We further demonstrate that the system...
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