Article
Barley stripe mosaic virus infection requires PKA-mediated phosphorylation of γb for suppression of both RNA silencing and the host cell death response.
The New phytologist - 1 Jun 2018
Zhang Xuan, Dong Kai, Xu Kai, Zhang Kun, Jin Xuejiao, Yang Meng, Zhang Yongliang, Wang Xianbing, Han Chenggui, Yu Jialin, Li Dawei
Abstract excerpt
The Barley stripe mosaic virus (BSMV) γb protein is a viral suppressor of RNA silencing (VSR) and symptom determinant. However, it is unclear how post-translational modification affects the different functions of γb. Here, we demonstrate that γb is phosphorylated at Ser-96 by a PKA-like kinase in vivo and in vitro. Mutant viruses containing a nonphosphorylatable substitution (BSMVS96A or BSMVS96R ) exhibited...
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