Article
Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a single-tube 'hanging droplet' PCR assay with modified pan-PV CODEHOP primers.
The Journal of general virology - 1 Jan 2018
Chouhy Diego, Kocjan Boštjan J, Staheli Jeannette P, Bolatti Elisa M, Hošnjak Lea, Sagadin Martin, Giri Adriana A, Rose Timothy M, Poljak Mario
Abstract excerpt
A modified pan-PV consensus-degenerate hybrid oligonucleotide primer (CODEHOP) PCR was developed for generic and sensitive detection of a broad-spectrum of human papillomaviruses (HPVs) infecting the cutaneous epithelium. To test the analytical sensitivity of the assay we examined 149 eyebrow hair follicle specimens from immunocompetent male patients. HPV DNA was detected in 60 % (89/149) of analysed eyebrow...
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