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Article

A method for single pair mating in an obligate parasitic nematode.

International journal for parasitology - 1 Feb 2018

Sargison Neil D, Redman Elizabeth, Morrison Alison A, Bartley David J, Jackson Frank, Naghra-van Gijzel Hardeep, Holroyd Nancy, Berriman Matthew, Cotton James A, Gilleard John S

Abstract excerpt

Parasitic nematode species have extremely high levels of genetic diversity, presenting a number of experimental challenges for genomic and genetic work. Consequently, there is a need to develop inbred laboratory strains with reduced levels of polymorphism. The most efficient approach to inbred line development is single pair mating, but this is challenging for obligate parasites where the adult sexual...

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PMID: 29111440
DOI: 10.1016/j.ijpara.2017.08.010

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