Article
Abiotic Stress Phenotyping of Polyamine Mutants.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Berberich Thomas, Sagor G H M, Kusano Tomonobu
Abstract excerpt
Plant mutants in polyamine pathway genes are ideal for investigating their roles in stress responses. Here we describe easy-to-perform methods for phenotyping Arabidopsis mutants under abiotic stress. These include measurements of root growth, chlorophyll content, water loss, electrolyte leakage, and content of the reactive oxygen species hydrogen peroxide (H2O2) and superoxide anion (O2-). Growth of Arabidopsis...
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