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TILLING in Brachypodium distachyon.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

de Bang Louise, Torp Anna Maria, Rasmussen Søren K

Abstract excerpt

TILLING is a low-cost screening method that allows for identification of mutations in a gene-of-interest within a range of few base pairs. TILLING can be applied to mutant populations or to plant collections of cultivars, landraces or crop wild relatives (Eco-TILLING). The method is based on the Cel1 enzyme cleavage of mismatches in PCR products amplified with labeled primers. The cleavage can be detected due to...

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PMID: 29039011
DOI: 10.1007/978-1-4939-7278-4_13

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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