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Detection and quantification of Spirocerca lupi by HRM qPCR in fecal samples from dogs with spirocercosis

19 Sept 2017

Abstract excerpt

BACKGROUND: Spirocerca lupi, the dog oesophageal nematode, causes a potentially fatal disease in domestic dogs, and is currently clinically diagnosed by coproscopy and oesophagoscopy. To date, a single molecular method, a semi-nested PCR, targeting the cox1 gene, has been developed to aid in the diagnosis of spirocercosis. The present study describes three novel high-resolution melt (HRM) quantitative PCR (qPCR)...

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PMID: 28927435
DOI: 10.1186/s13071-017-2374-3

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