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Semiquantitative Analysis of the Red, Dry, and Rough Colony Morphology of Salmonella enterica Serovar Typhimurium and Escherichia coli Using Congo Red.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Cimdins Annika, Simm Roger

Abstract excerpt

The Congo Red (CR) assay is a standard biofilm test assessing the colony morphology of bacteria growing on agar plates supplemented with the diazo dye Congo Red. Biofilm forming Salmonella enterica serovar Typhimurium and Escherichia coli produce a red, dry, and rough (rdar) morphotype on CR-plates. The phenotype is characterized by staining of the extracellular matrix components curli (brown color) and cellulose...

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PMID: 28889298
DOI: 10.1007/978-1-4939-7240-1_18

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