Article
Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate.
PloS one - 1 Jan 2017
Utsumi Yoshinori, Utsumi Chikako, Tanaka Maho, Ha Vu The, Matsui Akihiro, Takahashi Satoshi, Seki Motoaki
Abstract excerpt
Agrobacterium-mediated transformation is an important research tool for the genetic improvement of cassava. The induction of friable embryogenic callus (FEC) is considered as a key step in cassava transformation. In the present study, the media composition was optimized for enhancing the FEC induction, and the effect of the optimized medium on gene expression was evaluated. In relative comparison to MS medium,...
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