Article
Simultaneous silencing of isoamylases ISA1, ISA2 and ISA3 by multi-target RNAi in potato tubers leads to decreased starch content and an early sprouting phenotype.
PloS one - 1 Jan 2017
Ferreira Stephanus J, Senning Melanie, Fischer-Stettler Michaela, Streb Sebastian, Ast Michelle, Neuhaus H Ekkehard, Zeeman Samuel C, Sonnewald Sophia, Sonnewald Uwe
Abstract excerpt
Isoamylases hydrolyse (1-6)-alpha-D-glucosidic linkages in starch and are involved in both starch granule formation and starch degradation. In plants, three isoamylase isoforms with distinct functions in starch synthesis (ISA1 and ISA2) and degradation (ISA3) have been described. Here, we created transgenic potato plants with simultaneously decreased expression of all three isoamylases using a chimeric RNAi...
Topics
- Hexoses
- Isoamylase
- Phenotype
- Plant Leaves
- Plant Proteins
- Plant Tubers
- Plants, Genetically Modified
- Protein Isoforms
- RNA Interference
- RNA, Small Interfering
- Seedlings
- Solanum tuberosum
- Starch