Article
High-throughput format for the phenotyping of fungi on solid substrates.
Scientific reports - 27 Jun 2017
Cánovas David, Studt Lena, Marcos Ana T, Strauss Joseph
Abstract excerpt
Filamentous fungi naturally grow on solid surfaces, yet most genetic and biochemical analyses are still performed in liquid cultures. Here, we report a multiplexing platform using high-throughput photometric continuous reading that allows parallel quantification of hyphal growth and reporter gene expression directly on solid medium, thereby mimicking natural environmental conditions. Using this system, we have...
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