Article
Proteomic analysis of seminal plasma from locally-adapted "Curraleiro Pé-Duro bulls" (Bos taurus): identifying biomarkers involved in sperm physiology in endangered animals for conservation of biodiversity.
Animal reproduction science - 1 Aug 2017
Menezes E B, de Oliveira R V, van Tilburg M F, Barbosa E A, Nascimento N V, Velho A L M C S, Moreno F B, Moreira R A, Monteiro-Moreira A C O, Carvalho G M C, Ramos A F, Memili E, Moura A A
Abstract excerpt
The present study was aimed at evaluating the seminal plasma proteins and sperm parameters of Curraleiro Pé-Duro bulls. Semen was collected from 10 bulls by electroejaculation, and sperm parameters were evaluated in fresh and frozen-thawed semen. Seminal plasma proteins were analyzed by 2-D SDS-PAGE and mass spectrophotometry. Tools in computational biology were used to generate bioinformatic knowledge and...
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