Article
Simultaneous identification of Haemophilus influenzae and Haemophilus haemolyticus using real-time PCR.
Future microbiology - 1 Jun 2017
Price Erin P, Harris Tegan M, Spargo Jessie, Nosworthy Elizabeth, Beissbarth Jemima, Chang Anne B, Smith-Vaughan Heidi C, Sarovich Derek S
Abstract excerpt
AIM: To design a highly specific and sensitive multiplex real-time PCR assay for the differentiation of the pathogen Haemophilus influenzae from its nonpathogenic near-neighbor Haemophilus haemolyticus. MATERIALS & METHODS: A comparison of 380 Haemophilus spp. genomes was used to identify loci specific for each species. Novel PCR assays targeting H. haemolyticus (hypD) and H. influenzae (siaT) were designed....
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