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Identification of traditional Chinese medicinal pipefish and exclusion of common adulterants by multiplex PCR based on 12S sequences of specific alleles.

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis - 1 Apr 2018

Gao Linhui, Yin Yan, Li Jia, Yuan Yuan, Jiang Chao, Gao Wei, Zhang Xianan, Huang Luqi

Abstract excerpt

We aimed to establish a rapid and accurate allele-specific diagnostic Polymerase Chain Reaction (PCR) method for medicinal pipefish. To achieve this, pipefish genomic DNA was extracted, sequenced bi-directionally, and the data were analyzed. On this basis, specific identification primers were designed and a facile multiplex PCR system was established and optimized. Phylogenetic tree analysis showed that the six...

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PMID: 28129724
DOI: 10.1080/24701394.2016.1278538

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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