Pros and cons of direct genotyping on tuberculosis clinical samples.

doi:10.1016/j.micpath.2016.12.025

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Article

Pros and cons of direct genotyping on tuberculosis clinical samples.

Microbial pathogenesis - 1 Feb 2017

Sadegh Hamidreza, Kargarpour Kamakoli Mansour, Farmanfarmaei Ghazaleh, Masoumi Morteza, Abdolrahimi Farid, Fateh Abolfazl, Ebrahimzadeh Nayereh, Rahimi Jamnani Fatemeh, Vaziri Farzam, Siadat Seyed Davar

Abstract excerpt

OBJECTIVE: Prompt genotyping of Mycobacterium tuberculosis (M. tuberculosis) is crucial for improving molecular epidemiological investigation of tuberculosis (TB). METHODS: We performed a retrospective study to evaluate the use of 24 loci MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number of tandem-repeat) directly on 135 clinical samples from 84 TB patients. RESULTS: There was a direct...

Topics

Alleles
Bacterial Typing Techniques
DNA, Bacterial
Genetic Variation
Genotype
Genotyping Techniques
Humans
Minisatellite Repeats
Molecular Epidemiology
Mycobacterium tuberculosis
Retrospective Studies
Tuberculosis

Source

Open in PubMed

Identifiers

PMID: 28034830
DOI: 10.1016/j.micpath.2016.12.025

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