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Algorithm Optimization in Methylation Detection with Multiple RT-qPCR

29 Nov 2016

Abstract excerpt

Epigenetic markers based on differential methylation of DNA sequences are used in cancer screening and diagnostics. Detection of abnormal methylation at specific loci by real-time quantitative polymerase chain reaction (RT-qPCR) has been developed to enable high-throughput cancer screening. For tests that combine the results of multiple PCR replicates into a single reportable result, both individual PCR cutoff...

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PMID: 27898666
DOI: 10.1371/journal.pone.0163333

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