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Article

Sanger Confirmation Is Required to Achieve Optimal Sensitivity and Specificity in Next-Generation Sequencing Panel Testing.

The Journal of molecular diagnostics : JMD - 1 Nov 2016

Mu Wenbo, Lu Hsiao-Mei, Chen Jefferey, Li Shuwei, Elliott Aaron M

Abstract excerpt

Next-generation sequencing (NGS) has rapidly replaced Sanger sequencing as the method of choice for diagnostic gene-panel testing. For hereditary-cancer testing, the technical sensitivity and specificity of the assay are paramount as clinicians use results to make important clinical management and treatment decisions. There is significant debate within the diagnostics community regarding the necessity of...

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Identifiers

PMID: 27720647
DOI: 10.1016/j.jmoldx.2016.07.006

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