Article
Evaluation of a blood-specific DNA methylated region and trial for allele-specific blood identification from mixed body fluid DNA.
Legal medicine (Tokyo, Japan) - 1 Sept 2016
Watanabe Ken, Akutsu Tomoko, Takamura Ayari, Sakurada Koichi
Abstract excerpt
The identification of blood samples obtained from crime scenes has been an important step in forensic investigation. Recently, a novel approach using the blood-specific methylated CpG site cg06379435 has been reported. In this study, we developed a real-time polymerase-chain-reaction-based method that can simply and rapidly quantitate the methylation ratio of cg06379435 and its neighboring CpGs and set the...
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