Article
[A Detection of Allelic Variants at Microsatellite Markers by Using Capillary and Traditional Electrophoresis].
Genetika - 1 Apr 2016
Rubtsova G A, Ponomareva E V, Afanasiev K I, Shaikhaev E G, Kholodova M V, Pavlov S D, Zhivotovsky L A
Abstract excerpt
Microsatellite alleles are detected by PCR (polymerase chain reaction) that provides a manifold increase in the number of copies (amplification) of a given DNA fragment. The fragment visualization can be reached by two different methods. These are fragment analysis by capillary electrophoresis in denaturing gel and frag- ment separation in non-denaturing gel with subsequent gel staining. The first method is more...
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